Biofabrication and Cell Culture Laboratory

Competencies

Responsible Researcher: Nathalie Barroca

The Biofabrication and Cell Culture Laboratory (BioFAB) is a dedicated research space focused on biomaterials, biofabrication and tissue engineering. It is equipped with all the necessary resources to support every stage of work involving cells and biomaterials, from system preparation to biological evaluation.

The laboratory’s activities include the handling and maintenance of cell cultures under sterile conditions, using laminar‑flow hoods and CO₂ incubators, as well as the preparation of media and samples supported by ultracentrifuges and other auxiliary equipment. Culture monitoring is performed using an inverted microscope, enabling continuous assessment of cell viability and morphology over time.

The laboratory is also equipped with a 3D bioprinter (RegenHu Biodiscovery 3D Printer), which enables the production of complex biological structures in a sterile environment using customized bioinks. This capability supports the development of 3D tissue models for applications in regenerative medicine, in‑vitro systems and controlled drug‑delivery platforms.

Additionally, the LBCC features a patented 3D electrospinning system, allowing the production of micro‑ and nanofibres organized into complex architectures. This technology enables the engineering of biomimetic scaffolds with high structural resolution.

 

Equipments

Nikon Eclipse Optical Microscope

Model: Nikon – Eclipse LV150
Nikon Eclipse Optical Microscope

Electrospinning para deposição automatizada em 3D

Electrospinning para deposição automatizada em 3D

CO2 incubators (Binder - CB 220)

Model: Binder - CB 220
CO2 incubators (Binder - CB 220)

Cell counter (Beckman Coulter - Vi-Cell XR)

Model: Beckman Coulter - Vi-Cell XR
Cell counter (Beckman Coulter - Vi-Cell XR)

Biological safety cabinet (Class II laminar flow)

Model: Class II laminar flow

Autoclave (Kisker Biotech - Selecta Med 20)

Model: Kisker Biotech - Selecta Med 20
Autoclave (Kisker Biotech - Selecta Med 20)

Quartz crystal microbalance Q-Sense E4 (Biolin Scientific)

Model: Biolin Scientific
Quartz crystal microbalance Q-Sense E4 (Biolin Scientific)

Centrifuge (Multifuge Thermo Scientific X1)

Model: Multifuge Thermo Scientific X1

3D printer (Biodiscovery by RegenHu)

Model: Biodiscovery by RegenHu

Inverted optical microscope (OXION)

Model: OXION