Description
High-sensitivity microvolume spectrophotometer for rapid and accurate quantification of nucleic acids, proteins, and other biomolecules using minimal sample volumes.
Specifications:
Measurements
- Absorbance (UV-Vis): Minimum sample volume: ≥ 1 µL
- Fluorescence: Minimum sample volume: ≥ 2 µL
- Wavelength range: 190–850 nm
- Spectral bandwidth (FWHM): ≤ 1.8 nm
Accuracy and Sensitivity
- Photometric accuracy: ~3% at 0.97 Abs (302 nm) at 25 °C
- Typical repeatability: 0.002 Abs (1.0 mm pathlength) or CV ≤ 1% (10 measurements at 0.97 Abs)
- Wavelength accuracy: ± 1 nm
Measurement Ranges
- Photometric range (10 mm equivalent): Pedestal: 0–550 Abs
- Detection limit (dsDNA): Pedestal: ~1.0 ng/µL
- Proteins (BSA/IgG): Pedestal: ±0.03–0.015 mg/mL
